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Assays for Quantitation of HCV RNA in Serum and Their Dynamic Range

for Health Care Providers

Table 5. Assays for Quantitation of HCV RNA in Serum and Their Dynamic Range

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1 IU/mL = X copies/mL
Technique Dynamic Range (IU/mL)
Adapted from the AASLD Guidelines (17)
Roche Molecular Systems, Branchburg, NJ; Bayer Diagnostics, Tarrytown, NY; Abbot Diagnostics, Chicago, IL; National Genetics Institute, Los Angeles, CA.
AMPLICOR HCV Monitor V2.0 (Roche molecular systems)0.9 Manual competitive reverse
transcriptase PCR (rtPCR)
COBAS AMPLICOR HCV Monitor V2.0 (Roche molecular systems)2.7Semi-automated
competitive rtPCR
Versant HCV RNA 3.0 quantitative assay (Bayer diagnostics)5.2 Semi-automated 'branched DNA' assay 615-7,690,000
LCx HCV RNA quantitative assay (Abbot diagnostics) 3.8Semi-automated competitive rtPCR25-2,630,000
SuperQuant (National Genetics Institute)3.4Semi-automated competitive rtPCR 30-1,470,000